Effect of exogenous calcium on seed germination and seedling physiological characteristics of Lycium ruthenium / 中国中药杂志

<p><b>OBJECTIVE</b>In order to get the method for improving the salt resistance of Lycium ruthenium seeds and seedlings under NaCl stress, the seed germination and physiological characteristics of L. ruthenium seedlings was studied.</p><p><b>METHOD</b>Several physiological indexes of L. ruthenium seeds under NaCl stress, such as the germination rate (Gr), germination vigor (Gv), germination index (Gi), vigor index (Vi), and relative salt damage rate were measured. Other indexes of the seedlings like relative water contents (RWC) , chlorophyll contents, soluble protein contents, electrolyte leakage, the contents of malondialdehyde (MDA), and peroxidase (POD) were also measured.</p><p><b>RESULT</b>NaCl at lower concentration could promote the seed germination but inhibit the seed germination at higher concentration. After the treatment by CaCl2 at the different concentrations, all germination indexes were increased. With the increase of salt concentration, the relative water contents and the contents of chlorophyll were decreased, the content of MDA and electrolyte leakage were increased. The change trend of POD activity showed the first increase and then decrease with the increase of salt concentration, which was similar to that of the soluble protein. After the treatment by CaCl2, relative water contents, chlorophyll and POD activities were decreased more slowly, and also electrolyte leakage and MDA contents increased slowly.</p><p><b>CONCLUSION</b>The CaCl2 could significantly alleviate the damages to the seeds and seedlings of L. ruthenium under NaCl stress, and promote the salt resistance to the seeds and seedlings of L. ruthenium.</p>

Five alkaloids from vine stems of Diploclisia affinis / 中国中药杂志

<p><b>OBJECTIVE</b>To study alkaloid constituents of Diploclisia affinis.</p><p><b>METHOD</b>The air-dried vine stems of D. affinis were extracted with 90% EtOH three times at room temperature. The EtOH extract was suspended in H2 O and adjusted to pH 2 with 5% HCl solution. After extracted with petroleum ether and CHCl3 successively, the aqueous fraction was adjusted to pH 9 with 10% NH3 x H2O and extracted with CHCl3 again to afford the total alkaloids fraction. The compounds were isolated through column chromatography from the total alkaloids fraction. Their structures were determined on the basis of spectral analysis (MS, 1H-NMR, 13C-NMR).</p><p><b>RESULT</b>Five alkaloids was identified as reticuline (1), asimilobine (2), acutumine (3), dihydroxyprotoberberine (4), stepholidine (5), were isolated from the vine stems of D. affinis.</p><p><b>CONCLUSION</b>All the compounds were obtained from D. affinis for the first time.</p>